Callus induction was tried with explants such as nodes, internodes and leaves of freshly obtained twigs of Flacourtia jangomas with Murashige and Skoog (MS) media supplemented with different concentration of growth hormones like 2, 4-dichlorophenoxyacetic acid (2, 4-D) and Benzylaminopurine (BAP). The MS medium supplemented with 2, 4-D (2.0 mg/l) and BAP (0.5 mg/l) was found to be the most suitable for callus initiation and proliferation in case of nodes and internodes while for leaves MS medium containing 2, 4-D (1.5 mg/l) and BAP (0.5 mg/l) was found to be the best. In case of nodes and internodes percentage of callusing was maximum with media containing 2, 4-D (1.5 mg/l) and BAP (1.0 mg/l), while in case of leaves percentage of callusing was maximum in the same media, which was found suitable for initiation and proliferation.
Flacourtia jangomas (Lour.) Raeusch (Family Flacourtiaceae) is a woody, medicinal plant of dioecious habit, found frequently in semi-wild conditions in the Brahmaputra valley and adjoining areas in the Northeastern parts of India and had probably migrated from Bangladesh and upper Myanmar. The plant has medicinal as well as economic values and is mainly cultivated for its edible fruits and hard wood. The fruits are either eaten raw or used for making jams and preserves (Mitra, 1993; and Singh et al., 1994). Different parts of this plant are used for the treatment of asthma, pre- and post-natal blood purification in bilious conditions and diarrhoea (Kirtikar and Basu, 1953; and Jain, 1991). The plant contains tannin and a fixed oil and two limnoids (i.e., limolin and jangomolide) reported from its stem and bark (Ahmad et al., 1984; and Anon, 1986).
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