Possible Modulating Action of Plant Extract of Ocimum sanctum L. Against Methyl Methanesulphonate and Cyclophosphamide Induced Genotoxic Damage In Vitro
Article Details
Pub. Date
:
May, 2008
Product Name
:
The IUP Journal of Life Sciences
Product Type
:
Article
Product Code
:
IJLS40805
Author Name
:
Yasir Hasan Siddique, Gulshan Ara, Tanveer Beg and Mohammad Afzal
Availability
:
YES
Subject/Domain
:
Science & Technology
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:
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No. of Pages
:
9
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Description
Plants are used as a good source of medicines by the pharmaceutical industry. In the present study the action of Ocimum sanctum L. infusion on chromosomal aberrations (CAs), mitotic index (MI), Sister Chromatid Exchanges (SCEs) and Replication Index (RI) induced by Methyl Methanesulphonate (MMS) and Cyclophosphamide (CP) was assessed on human lymphocytes in vitro. The study was performed both in the absence as well as presence of metabolic activation (S9 mix).
The infusion at dosages of 1.075 x 10-4, 2.127 x 10-4 and 3.15 x 10-4 g/ml culture medium was given separately, along with 60 mM of MMS and 0.16 mg/ml of CP, respectively. The used dosage of plant infusions neither induced CAs and SCEs significantly nor affected RI and MI both in the absence as well as presence of metabolic activation (S9 mix). A dose dependent significant decrease in the genotoxic damage was observed at all the tested dosages of plant infusion when given along with 60 mM of MMS (without S9 mix) and 0.16 mg/ml of CP (with S9 mix). The results indicate that plant infusion per se does not have genotoxic activity but can modulate the genotoxic activity of MMS and CP on human lymphocytes in vitro.
