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The IUP Journal of Genetics & Evolution
Pollen and Spikelet Fertility Studies in Rice Crosses
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Eight CMS lines were crossed with 31 genotypes as `testers' to get 248 hybrids. The 248 hybrids were subjected to pollen and spikelet fertility analysis. Among the 248 hybrids, 168 hybrids were expressed as restorers, 52 as partial restorer (PR) and 28 as maintainers. Ten testers viz., IR 62037, IR 72865, IR 68427, MDU5, TP1021, RR363-1, RR 347-1, RR 286-1, ACK 99017 and ASD06-08 were identified as restorers for all the eight CMS lines.

 
 
 

The use of cytoplasmic genetic male sterility system in developing hybrids in crops is possible only when effective restorers are identified. The CMS lines introduced from China are unstable to use as such in developing hybrid rice in India. Therefore, it is imperative to identify maintainers and restorers among the lines developed through conventional breeding procedures. Pollen (or) spikelet fertility or both were used as an index to fix the restoration ability of the lines (Sutaryo, 1989). The present study was undertaken to find out the fertility restoration ability of 31 testers on eight CMS lines.

Materials and Methods

Eight cytoplasmic male sterile lines, viz., IR 80559 A (L1), APMS 2 A(L2), IR 72081 A(L3), IR 75601 A(L4), IR 75596 A(L5), IR 80154 A(L6), CRMS 32 A(L7), IR 75608 A (L8), and 31 testers, viz., IR 62124-83-3-2-1(T1), IR 62036-222-3-3-1-2(T2), IR 62037-93-1-3-1-1(T3), IR 63881-49-2-1-3-2(T4), IR 72865-94-3-3-2(T5), IR 62030-83-1-3-2(T6), IR 59673-93-2-3-3(T7), IR 68427-8-3-3-2(T8), IR 68926-61-2(T9), MDU 5(T10), ACK 99017(T11), AD 01259(T12), AD 01260(T13), TP 1021(T14), RR 363-1(T15), RR 361-3(T16), RR 354-1(T17), RR 347-1(T18), RR 348-6(T19), RR 286-1(T20), RR 166-645(T21), RR433-1(T22), RR 434-3(T23), ASD 06-1(T24), ASD 06-2(T25), ASD 06-3(T26), ASD 06-4(T27), ASD 06-5(T28), ASD 06-6(T29), ASD 06-7(T30) and ASD 06-8(T31), were raised in the LXT (8X31) mating design to get 248 hybrids during September 2007. Three staggered sowings were taken to synchronize the flowering.

 
 
 

Genetics & Evolution Journal, CMS lines, Restorers, Maintainers, Cytoplasmic Genetic, Cytoplasmic Source, Fertility-Restoring Genes, Cytoplasmic Interactions, Spikelet Fertility Analysis, Environmental Interaction.